What is the principle of spectrophotometry in laboratory tests?

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The principle of spectrophotometry in laboratory tests is based on the measurement of light absorbance to determine concentrations of substances. In this technique, a light beam passes through a solution containing the substance of interest. According to Beer-Lambert's Law, the amount of light absorbed by the solution is directly related to the concentration of the absorbing species in the solution and the path length of the light. By measuring the intensity of light before and after it passes through the sample, the concentration of the substance can be quantified.

This method is widely utilized in various fields, including clinical diagnostics, environmental monitoring, and biochemical research, due to its sensitivity and accuracy in analyzing different compounds. Understanding how much light is absorbed allows technologists to infer specific information about the samples being analyzed, making it a fundamental technique in laboratory settings.

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